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Photo of Winfried Moeller

Dr. Winfried Möller,

GSF National Research Center for Environment and Health, Institute of Inhalation Biology, Gauting
Helmholtz Zentrum München – German Research Center for Environmental Health Clinical Cooperation Group Inflammatory Lung Diseases

Curriculum Vitae

DATE AND PLACE OF BIRTH June 24th, 1957, Weidenau
FAMILY STATUS Married, 3 children
NATIONALITY German
CURRENT POSITION Senior Scientist
EDUCATION
1979 – 1985: Study of Physics at the Justus Liebig University Giessen (Germany), March 1985: Diploma in Applied Physics (Thesis in Superconductivity). 1988 – 1991: PhD study at the Institute for Biophysics, Johann Wolfgang von Goethe University, Frankfurt, Germany, April 1991 Dr. rer. nat. (Thesis in Aerosol Biophysics)
 
ACADEMIC APPOINTMENTS
1985 – 1988 Young scientist at the Institute for Biophysical Radiation Research, Gesellschaft für Strahlen und Umweltforschung (GSF), Frankfurt (Germany)
1991 – 1995: Post-doc at the Institute for Biophysical Radiation Research, Gesellschaft für Strahlen und Umweltforschung (GSF), Frankfurt (Germany)
1995 – today: Senior scientist at the Clinical Cooperation Group Inflammatory Lung Diseases, Helmholtz Zentrum München, Gauting, Germany.
 
MAJOR RESEARCH FIELDS
  • Generation and inhalation of magnetic and radiolabeled fine and ultrafine aerosols, study of particle deposition and clearance from the human respiratory tract.
  • Use of magnetic micro- and nanoparticles for studies of cytoskeleton dependent defence functions of macrophages, in vivo and in vitro (cytomagnetometry).
  • Nanoparticle toxicology of the cytoskeleton of macrophages.
  • Inflammatory response after targeted LPS inhalation.
  • Aerosolized drug delivery to the paranasal sinuses.
His Topic of Materials' Days 2009:

Relaxation and Twisting of Ingested Magnetic Micro- and Nanoparticles - an Assay for Cell Function and Cytoskeleton Mechanics.


Abstract:
In the lung macrophages from the major defence barrier against foreign substances, such as inhaled particles, bacteria or viruses. The initial step of this defence is ingestion (phagocytosis), followed by intracellular digestion and/or triggering of immune responses. The cytoskeleton plays a crucial role in this defence, since the cells have to be mobile, moving to the site of the invader. Magnetic micro- and nanoparticles are novel assays for studying these cytoskeleton dependent cell functions, in vitro and in vitro, and cytoskeletal dysfunctions have been monitored, as for example by co-culture with environmental nanoparticles and fibres. The magnetic tracers are ingested rapidly by macrophages, either in culture or after voluntary inhalation. In a strong magnetic field remanent magnetic dipoles are formed all being oriented parallel to the magnetizing field, allowing to detect them by sensitive magnetic field sensors (fluxgate or SQUID). Intracellular vesicle transport disturbs this order. This process called relaxation, requires energy (ATP) and an intact cytoskeleton and is therefore a monitor for cell viability and function. In addition application of twisting forces allows studying cytoskeletal mechanical properties, such as viscosity and elasticity. Experimental in vitro and in vivo data on relaxation and magnetic particles twisting are presented together with interpretation by mathematical models. In addition the influence of cytoskeletal drugs and cytotoxic nanoparticles are presented.